What are the functions of RNA polymerase I and II in eukaryotes?

What are the functions of RNA polymerase I and II in eukaryotes?

Eukaryotic cells contain three distinct nuclear RNA polymerases that transcribe different classes of genes (Table 6.1). Protein-coding genes are transcribed by RNA polymerase II to yield mRNAs; ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs) are transcribed by RNA polymerases I and III.

What is the purpose of DNA polymerase III?

The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis. Together with a DNA helicase and a primase, Pol III HE participates in the replicative apparatus that acts at the replication fork.

What does RNA polymerase II do?

RNA polymerase II (Pol II) transcribes all protein-coding genes and many noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it lacks the ability to initiate transcription and cannot consistently transcribe through long DNA sequences.

What are the two main differences between bacterial polymerase and eukaryotic RNA polymerase II function?

While there are similarities between prokaryotes and eukaryotes, there are many differences between them. Prokaryotes have only one RNA Polymerase, while eukaryotes have three (RNA Polymerases I, which transcribes rRNA; II, which transcribes mRNA; and III, which transcribes tRNA).

What are the main differences between prokaryotic DNA Polymerase I polymerase II and polymerase III?

DNA polymerase 1, 2 and 3 are prokaryotic DNA polymerases involved in DNA replication. Pol 1 catalyzes the repairing of DNA damages. Pol 2 catalyzes the fidelity and processivity of DNA replication. Pol 3 catalyzes the 5′ to 3′ DNA polymerization.

How is RNA polymerase used in RNA?

RNA Polymerase is used to generate specific RNA transcripts in vitrofrom DNA containing the T7 pro-moter sequence. The DNA sequence of interest is cloned into the polylinker region adjacent to the T7 promoter sequence of the vector. To prepare the DNA template for transcription, the recombinant plasmid is linearized at a restriction endonuclease

What are the two functions of RNA polymerase?

What are the two functions of RNA polymerase? RNA polymerases transcribe the information in DNA into RNA molecules that have a variety of functions, including messenger RNA, transfer RNA, ribosomal RNA (for protein synthesis), ribozymes (for catalysis), and microRNA (for regulation of gene expression).

What is the purpose of RNA polymerase?

RNA polymerase is said to be an enzyme that makes to take part in copying of the sequence in DNA and help in get converted to a sequence of RNA at the time of a method called transcription. It is same as that of the molecule that is made of the protein subunits and is much complex.

What are the different types of RNA polymerase?

RNA polymerase I,II and III are three types of eukaryotic RNA polymerases.

  • All enzymes are complex,multi-subunit proteins.
  • All enzymes are responsible for transcription.
  • All enzymes require a DNA template to produce RNA.
  • All enzymes require accessory protein factors for binding and initiation of the transcription.