What does Grocott stain?

What does Grocott stain?

Grocott methenamine silver (GMS) stain is commonly used for the identification of fungi on cytosmears and tissue sections. It imparts a black color to the fungal profiles and a pale green color to the background. It stains all pathogenic and nonpathogenic fungi and melanin.

What does Grocott hexamine-silver stain?

Chromic acid oxidation forms aldehydes from fungal cell wall polysaccharide components, which are subsequently demonstrated by reduction of an alkaline hexamine-silver complex. The reaction may be compared to that of the periodic acid Schiff reaction, (see PAS).

What is silver stain test?

Pseudomonas, Legionella, Leptospira, H. pylori, Bartonella and Treponema, and fungi such as Pneumocystis, Cryptococcus, and Candida are organisms that are stained with silver.

What is Mucicarmine stain used for?

The mucicarmine staining procedure is very specific in its detection of mucins of epithelial origin. It is often used to identify adenocarcinomas, distinguishing these from squamous-cell carcinomas. It is particularly useful in detecting adenocarcinomas originating from the gastrointestinal tract.

What does silver stain stain?

Silver stain formulations can be made such that protein bands stain black, blue-brown, red, or yellow, depending on their charge and other characteristics. This is particularly useful for differentiating overlapping spots on 2D gels.

What does GMS Stain detect?

In diagnostic labs, GMS tissue staining is often used in combination with microbiologic culture for diagnosis of fungal infections in people and animals, since the presence of fungal organisms in tissues indicates an invasive infection.

What is a silver stain used for?

Silver stain techniques are widely used to detect nanogram quantities of proteins following electrophoresis. Silver nitrate is the silver source in most silver stain procedures. Silver staining is a highly sensitive method for detecting proteins in polyacrylamide slab gels.

How sensitive is silver staining?

0.25-0.5 ng
Silver staining

Sensitivity Detection
Silver staining 0.25-0.5 ng Visual

What is a Steiner stain?

The modified Steiner stain is a non-specific silver stain for identifying bacteria in formalin-fixed, paraffin-embedded tissues. The principle behind its use is that bacteria are first sensitized using uranyl nitrate solution, making them able to precipitate silver from a silver nitrate solution.

Is H pylori urease positive?

INTRODUCTION. Helicobacter pylori is the best known bacterium showing urease activity, although many other bacteria carry this enzyme in different human ecosystems such as mouth and large bowel (1).