What is electroporation system?

Electroporation is a physical transfection method that uses an electrical pulse to create temporary pores in cell membranes through which substances like nucleic acids can pass into cells.

What is Eporator?

Features. The Eppendorf Eporator is a compact instrument designed for fast and controlled electroporation of bacteria, yeasts and other microorganisms. The instrument features an intuitive operation and user-friendly programming of standard methods.

What should be the voltage for electroporation to work?

Electroporation involves application of high voltage (>50 V) to create transient pores in the stratum corneum, leading to a large increase in drug transport, delivery of macromolecules, a quick drug effect onset, and insignificant or only minor skin damage (Golden et al., 1987; Regnier and Preat, 1998; Hofmann, 1999).

What cell type is used in electroporation?

It is non-viral, non-toxic and can be used on all cell types including mammalian, bacteria, algae, plant and yeast. It can be used on cells in all forms, in vitro or in vivo/ex vivo. In vitro is Latin for “within glass” and includes suspension cell, tissue slice/whole organ, and adherent cell.

What cell type is appropriate for electroporation?

Electroporation has been used successfully to deliver plasmid DNA to a variety of tissues in vivo (Heller et al., 2006a). Because of its physical nature, EP can be applied to practically any cell or tissue. Plasmid DNA in the appropriate diluent is injected into the tissue.

Who invented electroporation?

The technique was first described by Neumann et al. in 1982 [1] and is based on the earlier observation that biomembranes can be made transiently permeable when short electric impulses are applied [2].

What is Eppendorf eporator?

The Eppendorf Eporator is a compact instrument designed for fast and controlled electroporation of bacteria, yeasts and other microorganisms. The instrument features an intuitive operation and user-friendly programming of standard methods.

What is Eppendorf handling solutions?

Eppendorf Handling Solutions To make your job in the lab easier and more efficient – with this goal in mind we are developing products and solutions in the areas of Liquid Handling, Cell Handling, and Sample Handling.

What kind of support does Eppendorf offer?

Eppendorf Support Center To continue the highest standard of support for customers, Eppendorf has created an international Support Center. Pipette Calibration Done Right The foundation of reliable results. We offer a range of service options from basic calibration at an economical price to GLP/GMP custom-designed calibration services. Eppendorf

What’s new at myeppendorf previousnext?

myEppendorf PreviousNext New Centrifugation Solutions! Introducing Himac floor-standing centrifuges Introducing: epT.I.P.S.®Boxes 2.0! New upgraded design. Same great quality. Click to learn more. Done in a Snap Fast and safe sample handling with Eppendorf Conical Tubes 25 mL and SnapTec®50