What is meant by solid-phase synthesis in combinatorial synthesis?

What is meant by solid-phase synthesis in combinatorial synthesis?

A common combinatorial technique uses organic synthesis on solid support. Linker groups are attached to resin beads in the solid phase and the reactants are passed over in solution to make the intermediates and a final detachment-from-resin reaction is run to get the desired product out of the column.

Which synthesis solid support used in combinatorial chemistry?

Combinatorial split-mix (split and pool) synthesis is based on the solid-phase synthesis developed by Merrifield. If a combinatorial peptide library is synthesized using 20 amino acids (or other kinds of building blocks) the bead form solid support is divided into 20 equal portions.

What is meant by solid-phase synthesis?

Definition. Solid-phase synthesis is the synthesis of chemical compounds whereby the reactant molecule is chemically bound to an insoluble material and reagents are added in the solution-phase.

Which method is used in combinatorial synthesis?

Combinatorial chemistry uses chemical synthesis methods that make it possible to prepare a large number (up to even millions) of compositions in a single process. Combinatorial chemistry also includes strategies that allow identification of useful components of the libraries for such large-scale synthesis.

How is solid phase peptide synthesis?

Solid-phase peptide synthesis (SPPS) involves the successive addition of protected amino acid derivatives to a growing peptide chain immobilized on a solid phase, including deprotection and washing steps to remove unreacted groups and also side products.

What is parallel synthesis in combinatorial chemistry?

Parallel synthesis enables the rapid, logical synthesis of thousands of molecules, any one of which could provide a hit against the target entity. Prior to the use of parallel synthesis in combinatorial chemistry, the discovery of a lead molecules was a sequential process that was time consuming and/or fortuitous.

What do you mean by combinatorial synthesis?

From the stand point of the synthetic chemist, Combinatorial Synthesis involves the use of processes (solid phase synthesis) and strategies (hundreds or thousands of simultaneous reactions) which allow the preparation, in a short time, of big, organized collections of organic compounds, the so called molecular …

How can combinatorial chemistry is different than conventional synthesis?

(1) The creation of large libraries of molecules in a short time is the main advantage of combinatorial chemistry over traditional. (2)Compounds that cannot be synthesized using traditional methods of medicinal chemistry can be synthesized using combinatorial techniques.

What is solution phase synthesis?

Solution phase synthesis was the first developed and the only method for peptide synthesis until the solid phase peptide synthesis (SPPS) introduced by Merrifield revolutionized the way peptides and their analogues are prepared nowadays.

What is principle of combinatorial chemistry?

The basic principle of combinatorial chemistry is to prepare a large number of different compounds at the same time Instead of synthesizing compounds in a conventional one at a time manner and then to identify the most promising compound for further development by high throughput screening (Process that allows rapid …

What is solid phase synthesis?

Merrifield’s Solid Phase synthesis concept, first developed for biopolymer, has spread in every field where organic synthesis is involved. Many laboratories and companies focused on the development of technologies and chemistry suitable to SPS.

What is combinatorial synthesis?

A very remarkable combinatorial synthesis was developed by Fodor and his colleagues by combining the solid phase synthesis with the photolithographic procedure applied in the fabrication of the computer chips. The method was published in 1991 under the title “The light-directed, spatially addressable parallel chemical synthesis”.

How does solid phase combinatorial chemistry work?

In solid phase combinatorial chemistry, the starting compound is attached to an insoluble resin bead, reagents are added to the solution in excess, and the resulting products can be isolated by simple filtration, which traps the beads while the excess reagent is washed away. [Leard L. & Hendry A. (2007)]1

What is a split-mix synthesis?

The combinatorial “split-mix synthesis” is based on the solid-phase synthesis developed by Merrifield. If a combinatorial peptide library is synthesized using 20 amino acids (or other kinds of building blocks) the bead form solid support is divided into 20 equal portions.