What is the reason for using the beta actin antibody?

What is the reason for using the beta actin antibody?

Use Anti-beta-actin for detection of beta-actin by ELISA, immunohistology, western blotting, and immunofluorescence. The antibody specifically recognizes beta-actin in a wide variety of tissues and species using immunoblotting, immunofluorescent staining of cultured cell lines, and immunohistochemistry.

Why did we perform a PCR amplifying beta actin as a control along side our samples of interest?

Beta-Actin (42 kDa) is commonly chosen as a loading control due to its general expression across all eukaryotic cell types. The expression levels of this protein do not vary drastically due to cellular treatment, which is another reason the protein makes a suitable control.

How big is a beta-actin?

42 kDa
Though beta-actin has a molecular weight of 42 kDa, degradation by apoptosis will produce 38-41 kDa fragments on a Western blot.

Why is beta-actin a housekeeping gene?

GAPDH is a key regulatory enzyme, which catalyzes the oxidative phosphorylation of glyceraldehyde-3-phosphate during glycolysis. β-actin is a cytoskeletal protein involved in cell structure and motility. Based on these basic and ubiquitous cellular functions, GAPDH and β-actin are considered as housekeeping genes.

Where is beta-actin located?

Beta-actin

ACTB
Aliases ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin, beta, actin beta
External IDs OMIM: 102630 MGI: 87904 HomoloGene: 110648 GeneCards: ACTB
Gene location (Human) Chr. Chromosome 7 (human) Band 7p22.1 Start 5,526,409 bp End 5,563,902 bp

What does a loading control do?

A loading control is a protein used as a control in a Western blotting experiment. Typically, loading controls are proteins with high and ubiquitous expression, such as beta-actin or GADPH. They are used to make sure that the protein has been loaded equally across all wells.

What is the molecular weight of product a5441 antibody produced in mouse?

What is the molecular weight of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse? Beta Actin has a molecular weight of 42 kDa. Can I use Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse, to detect mouse beta actin?

What is monoclonal mouse anti Actin antibody?

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures. Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.

What is the molecular weight of beta actin?

Beta Actin has a molecular weight of 42 kDa. Can I use Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse, to detect mouse beta actin? This antibody will detect mouse beta actin.

Does actin cleavage show up on Western blot?

While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot.