What is meant by hydrodynamic radius?
What is meant by hydrodynamic radius?
The effective radius of an ion in a solution measured by assuming that it is a body moving through the solution and resisted by the solution’s viscosity. If the solvent is water, the hydrodynamic radius includes all the water molecules attracted to the ion.
How is hydrodynamic radius measured?
A value of the hydrodynamic radius can be calculated from measurements of the diffusion coefficient by means of the Stokes−Einstein equation. However, here we have used a different approach and have included a reference molecule in the protein solution ( 27).
What affects hydrodynamic radius?
For proteins, this is largely determined by the molecular weight, but shape also plays a role, with compact, well-folded proteins diffusing faster than extended, poorly folded proteins, and thus having a smaller hydrodynamic radius.
What is hydrodynamic radius of polymer?
Note that in biophysics, hydrodynamic radius refers to the Stokes radius, or commonly to the apparent Stokes radius obtained from size exclusion chromatography. The theoretical hydrodynamic radius. arises in the study of the dynamic properties of polymers moving in a solvent.
Why is hydrodynamic radius important?
The surface hydrophobicity (So), intrinsic viscosity (IV) and hydrodynamic radius (Rh) are important indicators of the structures of proteins, and can be used to evaluate the effect of processing on the denaturation and aggregation of proteins [12] .
What is hydrodynamic size in DLS?
Note that the diameter that is measured in DLS is a value that refers to how a particle diffuses within a fluid so it is referred to as a hydrodynamic diameter. The diameter that is obtained by this technique is the diameter of a sphere that has the same translational diffusion coefficient as the particle.
What is hydrodynamic volume?
Hydrodynamic volume – the volume of a polymer coil when it is in solution. This can vary for a polymer depending on how well it interacts with the solvent, and the polymer’s molecular weight.
What is hydrodynamic diameter of nanoparticles?
The hydrodynamic diameter for small particles increases from 7.1 ± 1.9 nm to 13.2 ± 3.3 nm as the MNP concentration increases from 25 to 50 mg/L.
What is the difference between hydrodynamic radius and radius of gyration?
The radius of gyration is typically calculated from static scattering measurements, while the hydro- dynamic radius (or equivalent sphere radius) is deter- mined by dynamic light scattering or other diffusion measurements.
What is the Z average?
The Z average is the intensity weighted mean hydrodynamic size of the ensemble collection of particles measured by dynamic light scattering (DLS).
What is K in Mark Houwink equation?
Mark-Houwink parameters used are “k” = 2.79 x 10-3 cm3/g and “a” = 1.2754 at 25°C, data obtained from Tinland and Rinaudo [25] (Dependence of the Stiffness of the Xanthan Chain on the External Salt Concentration.
What is the viscosity of polymer?
The measurement of the viscosity of polymer solutions in organic solvents gives us a value that is directly related to the molecular mass of the polymer. In other words, the higher the viscosity, the higher the molecular weight, although the correlation is not direct.
Note that in biophysics, hydrodynamic radius refers to the Stokes radius, or commonly to the apparent Stokes radius obtained from size exclusion chromatography. arises in the study of the dynamic properties of polymers moving in a solvent. It is often similar in magnitude to the radius of gyration.
What is the hydrodynamic radius of aerosol?
The mobility of non-spherical aerosol particles can be described by the hydrodynamic radius. In the continuum limit, where the mean free path of the particle is negligible compared to a characteristic length scale of the particle, the hydrodynamic radius is defined as the radius that gives the same magnitude of the frictional force,
How to configure the RADIUS server?
On the RADIUS server configure software distribution tokens. 2.2. Configure the RADIUS security information On the RADIUS server configure the ports and shared secret to be used. On the RADIUS server create a new user account for OTP probing. On the RADIUS server create user accounts synchronized with Active Directory accounts.
What ports are used by the RADIUS server?
The RADIUS server uses UDP ports for communication purposes, and each RADIUS vendor has its own default UDP ports for incoming and outgoing communication.