What is Wilson Blair medium?
What is Wilson Blair medium?
Selective agar introduced by WILSON-BLAIR (1927) for the isolation and differentiation of Salmonella typhi and other Salmonella species from clinical specimens, food and water. This medium complies with the recommendations of the United States Pharmacopeia.
Which bacteria grows on Wilson and Blair bismuth sulphite medium?
Bismuth Sulphite Agar is a modification of the original Wilson and Blair1 selective medium for the isolation and preliminary identification of Salmonella typhi and other salmonellae from pathological material, sewage, water supplies, food and other products suspected of containing these pathogens.
How does bismuth sulfite agar selective for Salmonella and Shigella species?
Bismuth sulfite and brilliant green are general inhibitors that hinder the growth of most bacteria while allowing salmonellae to grow. Ferrous sulfate utilization by Salmonella species results in H2S production and the formation of ferrous sulfide, an insoluble black precipitate.
How does bismuth sulfite agar work?
Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. BLBG and bismuth stop gram-positive growth. Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide.
What is Cary Blair medium?
Remel Cary Blair Transport Medium is a semisolid medium recommended for use in the transportation and preservation of clinical specimens, primarily stool and rectal swabs.
What is Wilson Blair Agar?
Wilson and Blair Agar was formulated by Wilson and Blair (1) for isolating Salmonella species especially Salmonella serotype Typhi from clinical specimens. Peptic digest of animal tissue and beef extract provide nitrogenous, carbonaceous compounds and other growth nutrients.
What is a common medium used to culture microorganisms?
The most common growth media for microorganisms are nutrient broths (liquid nutrient medium) or lysogeny broth medium. Liquid media are often mixed with agar and poured via a sterile media dispenser into Petri dishes to solidify. These agar plates provide a solid medium on which microbes may be cultured.
What is Wilson Blair agar?
Is BSA selective or differential?
Bismuth sulfite agar is selective due to the presence of inhibitors and is differential on the basis of hydrogen sulfide ( H2S) production.
What is Cary-Blair transport medium used for?
Remel Cary Blair Transport Medium is a semisolid medium recommended for use in the transportation and preservation of clinical specimens, primarily stool and rectal swabs. This medium has a low nutrient content to prevent replication of organisms while maintaining viability.
How does Cary-Blair prepare for medium?
Preparation of Cary-Blair transport medium:
- Suspend 12.6 grams in 991 ml distilled water.
- Heat to boiling to dissolve the medium completely.
- Cool to 50°C and aseptically add 9 ml of 1% aqueous calcium chloride solution.
- Adjust pH to 8.4 if necessary.
What is the modified Wilson and Blair medium?
A simple modification of Wilson & Blair’s medium for the isolation of typhoid and paratyphoid bacilli has been evolved. 1. Instead of the original six ingredients, this modification contains only three, namely, brilliant green, bismuth-ammonium-citrate and sodium sulphite, and may therefore be conveniently termed “B.B.S. medium”.
What is Wilson and Blair’s BBS agar used for?
Wilson and Blair’s BBS Agar formulated by Wilson and Blair (2) is recommended for isolating Salmonella species especially Salmonella Typhi from clinical specimens. The selective reagent formulation is a modification of the bismuth sulphite reagent described by Hajna and Perry (3).
What is Wilson Wilson & Blair ( 1931)?
Wilson, W. J. & Blair, E. M. McV. ( 1931 ). Further experience of the bismuth sulphite media in the isolation of Bacillus typhosus and B. paratyphosus from faeces, sewage and water. J.