Why are BHK-21 cells used?
The BHK-21 cells are useful for transformations and for stable and temporary transfections. BHK cells are also used to study viral infections.
What are BHK cells used for?
Baby hamster kidney (BHK) cells are most commonly used in the production of animal products, the most important of which is the production of a vaccine against Foot-and-Mouth Disease and rabies .
What are Huh7 cells?
HuH-7 (hereafter Huh7) is a permanent cell line established from male hepatoma tissue, which was surgically removed from a 57-year-old Japanese male in 1982 (Nakabayashi et al., 1982). Huh7 and its derivatives have been used as a convenient experimental substitute for primary hepatocytes.
What type of cells are HeLa cells?
HeLa cells are a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken in 1951 from Henrietta Lacks, a patient who eventually died of her cancer.
How do 293T cells grow?
Place the cells in the 37 °C incubator with 5% CO2. 6. Gently replace medium after 24 hours with 14 mL HEK293T Culture Medium and continue to culture at 37 ºC with 5% CO2. Cells should be ready for passage or expansion to a T175 flask in ~ 2-3 days.
What are MRC 5 cells?
MRC-5 is the most common human diploid cell line used in production of viral vaccines; mesenchymal stem cells (MSCs) is a type of adult multipotent stem cells. Both cell types share the same fibroblast-like morphology and maintain a normal diploid karyotype over long in vitro expansion.
What does BHK-21 stand for?
A serum-free medium for serial culture of baby hamster kidney cell line 21 (BHK-21) as container-adherent cells was developed.
What are the BHK-21 cells resistant to?
BHK-21 cells are resistant to poliovirus 2 and Rabbit vesivirus (RaV) . The cells are negative for reverse transcriptase, which means that they lack integral retrovirus genomes. The BHK-21 cells are useful for transformations and for stable and temporary transfections.
What is the origin of BHK-21 fibroblast cell line?
The BHK-21 fibroblast cell line was established in March of 1961 by I. A. Macpherson and M. G. P. Stoker. The widely used line is a subclone (clone 13) of a parental line derived from the kidneys of five unsexed, 1-day-old hamsters. These hamsters were of the species Mesocricetus auratus and are commonly known as Syrian golden hamsters.
What is BHK cell line?
Baby Hamster Kidney fibroblasts ( BHK cells) are an adherent cell line used in molecular biology . The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters .